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| 购买进口仪器、试剂和耗材——就在始于2001年的毕特博生物 www.bitebo.com |
最专业有效的细胞离心涂片系统 Tharmac® Cellspin 载玻片离心机 (点击标题进入） 细胞治疗所使用的无血清培养基 lonza 04－418Q 无血清培养基 (点击标题进入） 干细胞治疗所使用的无血清培养基lonza 12-725F 无血清培养基 (点击标题进入）
抗逆转录病毒疗法不能治愈HIV-1感染：尽管接受了药物治疗，但大部分患者体内仍有大量处于休眠状态的这种病毒，即存在病毒库。 对从30位被HIV-1感染的患者(他们持续接受抗逆转录病毒治疗至少两年时间并且血浆中的HIV-1 RNA一直保持在检测不到的水平)分离出的免疫细胞所做的这项研究显示，这些病毒库以具有“细胞毒性T-淋巴细胞逃避突变”的病毒为主导。 该发现表明，治疗性疫苗设计的未来方向也许需要关注怎样提升大范围的“细胞毒性T-淋巴细胞反应”。 原文链接： Broad CTL response is required to clear latent HIV-1 due to dominance of escape mutations Despite antiretroviral therapy (ART), human immunodeficiency virus (HIV)-1 persists in a stable latent reservoir1, 2, primarily in resting memory CD4+ T cells3, 4. This reservoir presents a major barrier to the cure of HIV-1 infection. To purge the reservoir, pharmacological reactivation of latent HIV-1 has been proposed5 and tested both in vitro and in vivo6, 7, 8. A key remaining question is whether virus-specific immune mechanisms, including cytotoxic T lymphocytes (CTLs), can clear infected cells in ART-treated patients after latency is reversed. Here we show that there is a striking all or none pattern for CTL escape mutations in HIV-1 Gag epitopes. Unless ART is started early, the vast majority (>98%) of latent viruses carry CTL escape mutations that render infected cells insensitive to CTLs directed at common epitopes. To solve this problem, we identified CTLs that could recognize epitopes from latent HIV-1 that were unmutated in every chronically infected patient tested. Upon stimulation, these CTLs eliminated target cells infected with autologous virus derived from the latent reservoir, both in vitro and in patient-derived humanized mice. The predominance of CTL-resistant viruses in the latent reservoir poses a major challenge to viral eradication. Our results demonstrate that chronically infected patients retain a broad-spectrum viral-specific CTL response and that appropriate boosting of this response may be required for the elimination of the latent reservoir.(doi:10.1038/nature14053) HIV: Seeking ultimate victory HIV variants that have mutated to escape T-cell immune responses dominate the latent viral reservoir in most patients on antiretroviral therapy. This finding will need to guide therapeutic approaches targeting reactivated virus. (doi:10.1038/nature14194) 对应Nature杂志： 2015年01月15日Nature杂志精选
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购买进口仪器、试剂和耗材——就在始于2001年的毕特博生物
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