产品描述:
FlashGel® System for Recovery
5分钟DNA回收
产品介绍:
FlashGel® System for Recovery
5分钟DNA回收
利用回收的FlashGel ®系统直接回收DNA无需制备琼脂糖凝胶,带切除和纯化。该系统可提供高回收率,
无抑制剂和紫外线损害,只需5-10min的简单操作。
•从上样到回收只需5min
•直接从cassette回收样品,不需切除和纯化
•在电泳槽里可视化的样品回收,不需紫外线照射,80% - 100%的回收率
快速简单的程序
1)在顶行的小孔加入样品
2)电泳,直到样品到第二行孔处(2-5min)
3)停止电泳,并加入FlashGel ®回收缓冲液
4)从孔里吸取DNA
该FlashGel ®回收系统无需切胶和纯化。DNA迁移到第二行孔时,借助FlashGel ®缓冲液通过吸液管方便
地提取。样品回收率达80% - 100%,摆脱抑制剂和可用于下一步扩增,克隆或其他技术,均不需额外
的纯化步骤。
要开始使用回收,需订购FlashGel ®回收试剂盒(Cat. No. 57050)
货号
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描述
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规格
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57051
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FlashGel ® 回收Cassettes-9/pk
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1.2%agarose, 8+1 double-tier
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57060
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FlashGel ® 回收缓冲液 ready-to-use
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2 x 500ul
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50475
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FlashGel ®QuantLadder 100bp(3ng)-1.5kb(30ng) ready-to-load 50次
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250ul
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57050
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FlashGel ®回收Starter Kit 含回收Cassettes,回收缓冲液, QuantLadder, Loading Dye, 可视化的玻璃罩和对照.
Dock需单独购买
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Each
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5分钟的DNA回收
Direct DNA recovery using The FlashGel® System for Recovery eliminates agarose gel
preparation, band excision and purification. The system delivers highly efficient recovery,
free from inhibitors and UV-induced damage, in a simple 5-10 minute protocol.
- Go from sample loading to recovery in just 5 minutes
- Recover samples directly from the cassette, without band excision or purification
- Visualize sample recovery at the bench, without UV light Recover at 80% - 100%
efficiency
快速简单的程序
1. Load samples in top tier of wells
2. Run until samples reach the second tier of wells (2-5 minutes)
3. Stop the run and add FlashGel® Recovery Buffer
4. Remove the DNA from the wells
The FlashGel® System for Recovery eliminates the need to cut away and then purify bands.
As DNA migrates to the second tier of wells, it is free from the agarose matrix and easily
extracted via pipette, with the aid of the FlashGel® Recovery Buffer. Samples are recovered
at 80% - 100% efficiency, free from inhibitors and ready for subsequent re-amplification, cloning
or other techniques, without additional purification steps.
The FlashGel® System (Cat. No. 57067) contains a FlashGel® Dock, a FlashGel® Camera, a pack
of 1.2% DNA Cassettes, Loading Dye and Marker. To get started with recovery, order the FlashGel®
Recovery Kit (Cat. No. 57050), which contains 1.2% Recovery Cassettes, Recovery Buffer,
QuantLadder, Visualization Glasses and a Control fragment.
许可信息
Some components and technology of the FlashGel® System are sold under licensing agreements.
The nucleic acid stain in this product is manufactured and sold under license from Molecular Probes,
Inc., for use only in research applications or quality control, and is covered by pending and issued
patents. The FlashGel® Dock technology contains Clare Chemical Research, Inc. Dark Reader®
transilluminator technology and is covered under US Patents 6,198,107; 6,512,236; and 6,914,250.
The electrophoresis technology is licensed from Temple University and is covered under US Patent
6,905,585.
闪胶回收系统订购信息
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